RNA polymerase synthesizes a daughter strand of RNA in the _____ direction.

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Multiple Choice

RNA polymerase synthesizes a daughter strand of RNA in the _____ direction.

Explanation:
RNA polymerase builds the RNA chain by adding nucleotides to the 3' end, so synthesis occurs in the 5' to 3' direction. It reads the DNA template strand in the 3' to 5' direction, ensuring the RNA is complementary and grows toward the 3' end. This chemistry requires a free 3' hydroxyl and incoming ribonucleoside triphosphates to form the phosphodiester bond. Directions like 3' to 3' or 5' to 5' aren’t feasible for polymerase activity because nucleotide addition must happen at the 3' end, making 5' to 3' the correct orientation.

RNA polymerase builds the RNA chain by adding nucleotides to the 3' end, so synthesis occurs in the 5' to 3' direction. It reads the DNA template strand in the 3' to 5' direction, ensuring the RNA is complementary and grows toward the 3' end. This chemistry requires a free 3' hydroxyl and incoming ribonucleoside triphosphates to form the phosphodiester bond. Directions like 3' to 3' or 5' to 5' aren’t feasible for polymerase activity because nucleotide addition must happen at the 3' end, making 5' to 3' the correct orientation.

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